Premium
The non‐human primate kidney transcriptome in fetal development
Author(s) -
SpradlingReeves Kimberly D.,
Glenn Jeremy P.,
Lange Kenneth J.,
Kuhn Natalia,
Coalson Jacqueline J.,
Nijland Mark J.,
Li Cun,
Nathanielsz Peter W.,
Cox Laura A.
Publication year - 2018
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12340
Subject(s) - transcriptome , biology , kidney development , context (archaeology) , gene , gene expression , kidney , baboon , alternative splicing , fetus , primate , gene cluster , biological pathway , computational biology , genetics , bioinformatics , messenger rna , pregnancy , neuroscience , endocrinology , paleontology , embryonic stem cell
Background Little is known about the repertoire of non‐human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation. Methods The baboon kidney transcriptome was characterized at 60‐day gestation ( DG ), 90 DG , 125 DG , 140 DG , 160 DG and adulthood (6‐12 years) using gene arrays and validated by QRT ‐ PCR . Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways. Results Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development. Conclusions This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.