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Molecular genetic identification of isolates of the hepatitis A virus (HAV) from monkeys at Adler Primate Center
Author(s) -
Dogadov Dmitriy I.,
Korzaya Lydia I.,
Karlsen Anastasia A.,
Kyuregyan Karen K.
Publication year - 2018
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12333
Subject(s) - virology , primate , biology , hepatitis a virus , identification (biology) , virus , ecology
Background The aim of this study was to assess prevalence and genetic variability of hepatitis A virus (HAV) isolates in monkeys born and kept at Adler Primate Center, as well as in imported animals. Methods The fecal samples from various species of monkeys (n = 119) were studied using reverse transcription seminested PCR, sequencing, and phylogenetic analysis. Results HAV RNA was detected in 2 Macaca mulatta and 1 Macaca fascicularis (3.8%) kept at Adler Primate Center (n = 79) and in 11 (27.5%) Chlorocebus pygerythrus (n = 40) imported from Tanzania. Phylogenetic analysis demonstrated that all HAV strains belonged to simian genotype V, but differed from the prototype genotype V strain (AGM‐27) by 5.4%‐5.5%. Sequences isolated in this study differed by only 0.1%, suggesting a common source of infection. Conclusions This study demonstrated the asymptomatic circulation of HAV genotype V among the monkeys at Adler Primate Center, and it indicated the significant genetic diversity within this HAV genotype.