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Detection of Mycobacterium avium subsp. paratuberculosis in non‐human primates
Author(s) -
Fechner Kim,
MätzRensing Kerstin,
Lampe Karen,
Kaup FranzJosef,
Czerny ClausPeter,
Schäfer Jenny
Publication year - 2017
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12270
Subject(s) - paratuberculosis , ileum , biology , marmoset , mycobacterium , bone marrow , pathology , genetics , medicine , immunology , paleontology , bacteria , endocrinology
Background Due to a sporadic occurrence of Mycobacterium avium subsp. paratuberculosis ( MAP ) in non‐human primates ( NHP ), the susceptibility of different NHP to MAP should be investigated. Methods Fecal and tissue samples (ileum, ileocecal lymph node, bone marrow) of 20 animals (seven species) were analyzed by IS 900‐based PCR s and sequenced. Samples of MAP PCR positive NHP were further cultivated. Results MAP DNA was detectable in two animals; the ileum of a cottontop tamarin and the bone marrow of a common marmoset. Cultivation of MAP failed. Sequence analysis revealed 100% homology to the MAP ‐K10 sequence. Pathohistological examinations offered no direct correlation to a MAP infection. Conclusions MAP was detected for the first time in a common marmoset. But as both NHP suffered from other diseases, an asymptomatic infection with MAP was assumed. The detection of MAP in the bone marrow might play a role in establishing latent paratuberculosis, as known from tuberculosis.