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Evidence of simian retrovirus type D by polymerase chain reaction
Author(s) -
Hwa Christian Z.R.,
Tsai Sheung Pun,
Yee JoAnn L.,
Van Rompay Koen K.,
Roberts Jeffrey A.
Publication year - 2017
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12266
Subject(s) - virology , polymerase chain reaction , biology , nested polymerase chain reaction , macaque , retrovirus , multiplex polymerase chain reaction , rhesus macaque , multiplex , simian , microbiology and biotechnology , antibody , virus , gene , genetics , paleontology
Background Over the past few years, there have been reports of finding Simian retrovirus type D ( SRV ) in macaque colonies where some animals were characterized as antibody positive but virus negative raising questions about how SRV was transmitted or whether there is a variant strain detected by antibody but not polymerase chain reaction ( PCR ) in current use. Methods We developed a three‐round nested PCR assay using degenerate primers targeting the pol gene to detect for SRV serotypes 1‐5 and applied this newly validated PCR assay to test macaque DNA samples collected in China from 2010 to 2015. Results Using the nested PCR assay validated in this study, we found 0.15% of the samples archived on FTA ® cards were positive. Conclusions The source of SRV infection identified within domestic colonies might have originated from imported macaques. The multiplex nested PCR assay developed here may supplement the current assays for SRV .