Premium
Quantification of dynamic changes to blood volume and vascular flow in the primate corpus luteum during the menstrual cycle
Author(s) -
Bishop Cecily V.,
Molskness Theodore A.,
Xu Fuhua,
Belcik J. Todd,
Lindner Jonathan R.,
Slayden Ov D.,
Stouffer Richard L.
Publication year - 2014
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12132
Subject(s) - luteal phase , corpus luteum , ovary , medicine , menstrual cycle , endocrinology , blood flow , biology , andrology , follicular phase , hormone
Background The objective of the current study was to determine changes to vascular parameters of nonhuman primate dominant ovarian structures by dynamic contrast‐enhanced ultrasound ( DCE ‐ US ). Materials and Methods Dynamic contrast‐enhanced ultrasound with intravenous microbubble infusion was performed on the rhesus macaque ovary bearing the pre‐ovulatory follicle and corpus luteum ( CL ) sequentially during the natural luteal phase (n = 8) and G n RH antagonist (antide)‐induced luteal regression (n = 6). Results Changes in luteal blood volume ( BV ) and vascular flow ( VF ) were observed between stages of the luteal phase Luteal BV was highest in early stage CL , before decreasing 2.5‐fold in late stage CL ( P < 0.06); in contrast, luteal VF peaked at mid luteal stage ( P < 0.01). Two females identified with luteal insufficiency trended toward lower peak BV , compared to typical CL s. Another female was identified with a luteal cyst on the contralateral ovary, and a CL that regressed before P levels declined. After 72 hours of antide exposure, BV was reduced 2.3‐fold ( P = 0.03). Conclusions DCE‐US provides a sensitive, non‐invasive measurement of the dynamics of blood volume and flow in dominant ovarian structures.