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Primates karyological diagnosis and management programs applications
Author(s) -
Steinberg Eliana R.,
Nieves Mariela,
Fantini Lucía,
Mudry Marta D.
Publication year - 2014
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12131
Subject(s) - biology , captivity , zoology , fluorescence in situ hybridization , karyotype , evolutionary biology , genome , fish <actinopterygii> , chromosome , genetics , gene , fishery
Abstract Background Captive primates are often maintained in groups without geographic origin or genetic heritage information. This could lead to an incorrect assignment of species, which could result in an inadequate management of the colonies. Methods We present a cytogenetic protocol adapted to be successfully used in an accurate taxonomic diagnosis of non‐human primates (Platyrrhini), including lymphocyte culture, G‐ and C‐banding, meiosis, and fluorescent in situ hybridization technique ( FISH ). Results Using classical cytogenetic diagnosis, the species status was determined in 541 Platyrrhini individuals. Of these, 99 were previously erroneously sexed or assigned to a different species using only morphological characteristics. Conclusions The cytogenetic results highlight the relevance of the genetic characterization of primates both in captivity and in the wild. These techniques had been used in our research group for more than 30 years in different research projects, not only for characterizing hundreds of primates, but also different for topics regarding primates genomes and evolution.