Characterization of γδ T cells in naïve and HIV ‐infected chimpanzees and their responses to T ‐cell activators in vitro
Author(s) -
Hodara Vida L.,
Parodi Laura M.,
Chavez Deborah,
Smith Lisa M.,
Lanford Robert,
Giavedoni Luis D.
Publication year - 2014
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12115
Subject(s) - flow cytometry , biology , immune system , t cell , immunology , interleukin 21 , cytokine , in vitro , cytotoxic t cell , microbiology and biotechnology , virology , genetics
Abstract Background γδ T cells are effector cells that eliminate cancer and virus‐infected cells. Chimpanzees are an endangered species that can naturally and experimentally be infected with SIV and HIV , respectively, but no information about the functionality of γδ T cells during chronic lentiviral infection is currently available. Methods Healthy and HIV ‐infected chimpanzee γδ T cells were characterized by flow cytometry. γδ T subsets were studied after stimulation with T ‐cell activators, and the release of cytokines was analyzed by L uminex assay. Results γδ T ‐cell subsets, V δ1 and V δ2 V γ9, showed different patterns in the expression of CD 4, CD 195, CD 159a, and CD 159c. Stimulation of γδ T cells resulted in increased levels of CD 4 and HLA ‐ DR , which is more pronounced in V δ1 T cells. Distinct cytokine patterns were found between healthy and HIV ‐infected chimpanzees. Conclusions Analyses of major chimpanzee γδ T subsets show similarities to human γδ T cells and suggest different functionality and roles in their immune response against HIV infection.