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Development of a novel rhesus macaque model with an infectious R 5 simian–human immunodeficiency virus encoding HIV ‐1 CRF 08_ BC env
Author(s) -
Huang Li,
Kusagawa Shigeru,
Zeng Haiyan,
Yang Guibo,
Sun Binlian,
Miura Tomoyuki,
Yang Rongge
Publication year - 2014
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12084
Subject(s) - simian immunodeficiency virus , virology , rhesus macaque , macaque , biology , peripheral blood mononuclear cell , clone (java method) , virus , simian , immunology , gene , in vitro , genetics , paleontology
Background The CRF 08_ BC strain is one of the most predominant circulating Human immunodeficiency virus type 1 ( HIV ‐1) strains in the Chinese pandemic. A simian–human immunodeficiency virus ( SHIV ) encoding HIV ‐1 CRF 08_ BC env is highly desirable to evaluate candidate AIDS vaccines in non‐human primates. Methods SHIV ‐ KBQJ ‐12, which carries the envelope glycoprotein from QJ 001, an infectious molecular clone of HIV ‐1 CRF 08_ BC , was generated. The replication capacity of SHIV ‐ KBQJ ‐12 was determined both in human and rhesus macaque ( M acaca mulatta ) peripheral blood mononuclear cells ( PBMC s) and in C hinese rhesus macaques. Results SHIV ‐ KBQJ ‐12 replicated efficiently in human and macaque PBMC s and displayed a preference for CCR 5 as an entry coreceptor. Productive infection of two macaques by intravenous inoculation with SHIV ‐ KBQJ ‐12 was confirmed. Conclusions SHIV ‐ KBQJ ‐12 is an R 5‐tropic chimeric virus that can establish productive infection both in vitro and in vivo in C hinese rhesus macaques and will be useful to assess candidate HIV ‐1 CRF 08_ BC vaccines in C hina.