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Enhancing genotyping of MAOA‐ LPR and 5‐HTT‐ LPR in rhesus macaques ( Macaca mulatta )
Author(s) -
Karere Genesio M.,
Sullivan Erin,
Kinnally Erin L.,
Capitanio John P.,
Lyons Leslie A.
Publication year - 2012
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1111/jmp.12024
Subject(s) - genotyping , monoamine oxidase a , capillary electrophoresis , concordance , genotype , biology , amplicon , microbiology and biotechnology , genetics , serotonin , polymerase chain reaction , gene , receptor
Background Genetic variation in monoamine oxidase A ( MAOA ) and serotonin transporter ( 5‐ HTT )‐linked polymorphic regions ( LPR ) is associated with neuropsychiatric behavior. Methods We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. Results Genotype concordance was 97% and 95% for MAOA ‐ LPR and 5‐ HTT ‐ LPR , respectively. Capillary electrophoresis was more sensitive and cost‐effective. Conclusions Multiplexing MAOA ‐ LPR and 5‐ HTT ‐ LPR will enhance the genotyping of large sample sets.