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The effects of measurement parameters on the cancerous cell nucleus characterisation by atomic force microscopy in vitro
Author(s) -
Zhu Jiajing,
Tian Yanling,
Yan Jin,
Hu Jing,
Wang Zuobin,
Liu Xianping
Publication year - 2022
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.13104
Subject(s) - atomic force microscopy , indentation , force spectroscopy , lung cancer , a549 cell , materials science , moduli , biophysics , elastic modulus , cancer cell , cancer , chemistry , biomedical engineering , nanotechnology , pathology , medicine , composite material , biology , physics , quantum mechanics
Cancer is now responsible for the major leading cause of death worldwide. It is noteworthy that lung cancer has been recognised as the highest incidence (11.6%) and mortality (18.4%) for combined sexes among a variety of cancer diseases. Therefore, it is of great value to investigate the mechanical properties of lung cancerous cells for early diagnosis. This paper focus on the influence of measurement parameters on the measured central Young's moduli of single live A549 cell in vitro based on the force spectroscopy mode of atomic force microscopy (AFM). The effects of the measurement parameters on the measured central Young's moduli were analysed by fitting the force–depth curves utilising the Sneddon model. The results revealed that the Young's moduli of A549 cells increased with the larger indentation force, higher indentation speed, less retraction time, deeper Z length and lower purity percentage of serum. The Young's moduli of cells increased first and then decreased with the increasing dwell time. Hence, this research may have potential significance to provide reference for the standardised detection of a single cancerous cell in vitro using AFM methodologies.