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Simultaneous multicolour imaging using quantum dot structured illumination microscopy
Author(s) -
ZENG HUI,
YANG HUAIDONG,
LIU GUOXUAN,
ZHANG SICHUN,
ZHANG XINRONG,
ZHANG YINXIN
Publication year - 2020
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12862
Subject(s) - quantum dot , resolution (logic) , physics , microscopy , optics , wavelength , fluorescence , excitation , optoelectronics , computer science , artificial intelligence , quantum mechanics
Summary Multicolour structured illumination microscopy (SIM) is a powerful tool used for the investigation of the dynamic interaction between subcellular structures. Nevertheless, most of the multicolour SIM schemes are currently limited by conventional fluorescent dyes and wavelength‐dependent optical systems, and can only sequentially record images of different colour channels instead of obtaining multicolour datasets simultaneously. To address these issues, we present a novel multicolour SIM scheme referred to as quantum dot structured illumination microscopy (QD‐SIM). QD‐SIM enables simultaneously excitation and collection of multicolour fluorescent signals. We also propose a theoretical analysis of the image formation in two‐dimensional multicolour SIM to help combine the optically sectioned and super‐resolution attributes of SIM. Based on this theory, QD‐SIM enables optically sectioned, super‐resolution, multicolour simultaneous imaging at a single plane.