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Displacement fields using correlation methods as a tool to investigate cell migration in 3D collagen gels
Author(s) -
FERTIN ARNOLD,
LAFORGUE LAURE,
DUPERRAY ALAIN,
LAURENT VALÉRIE M.,
USSON YVES,
VERDIER CLAUDE
Publication year - 2019
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12825
Subject(s) - displacement (psychology) , materials science , chemistry , psychology , psychotherapist
Summary Living cells embedded in a complex extra‐cellular matrix migrate in a sophisticated way thanks to adhesions to matrix fibres and contractility. It is important to know what kind of forces are exerted by the cells. Here, we use reflectance confocal microscopy to locate fibres accurately and determine displacement fields. Correlation techniques are used to this aim, coupled with proper digital image processing. Benchmark tests validate the method in the case of shear and stretching motions. Finally, the method is tested successfully for studying cancer cells migrating in collagen gels of different concentration.