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Second‐harmonic imaging microscopy for identifying colorectal intraepithelial neoplasia
Author(s) -
LI L.H.,
JIANG W.Z.,
KANG D.Y.,
LIU X.,
LI H.S.,
GUAN G.X.,
ZHUO S.M.,
CHEN Z.F.,
CHEN J.X.
Publication year - 2018
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12690
Subject(s) - lamina propria , pathology , intraepithelial neoplasia , medicine , precancerous condition , high grade prostatic intraepithelial neoplasia , microscopy , epithelium , cancer , prostate
Summary In this study, second‐harmonic imaging microscopy was used to monitor precancerous colorectal lesions at different stages. It was found that the morphology of glands and lamina propria in mucosa changes with the progression of colorectal diseases from normal to low‐grade intraepithelial neoplasia to high‐grade intraepithelial neoplasia and this microscopy has the ability of direct visualization of these warning symptoms. Furthermore, two morphologic variables were quantified to determine the changes of glands and collagen in lamina propria during the development of colorectal intraepithelial neoplasia. These results suggest that second‐harmonic imaging microscopy has the potential in label‐freely and effectively distinguishing between normal and precancerous colorectal tissues, and will be helpful for early diagnosis and treatment of colorectal diseases.