Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography | Zendy

HAFFNERSTATON E. | Zendy; LA ROCCA A. | Zendy; FAY M.W. | Zendy

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Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

Author(s) -

HAFFNERSTATON E.,

LA ROCCA A.,

FAY M.W.

Publication year - 2018

Publication title -

journal of microscopy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.569

H-Index - 111

eISSN - 1365-2818

pISSN - 0022-2720

DOI - 10.1111/jmi.12680

Subject(s) - soot , characterization (materials science) , electron tomography , tomography , throughput , process engineering , nanoparticle , computer science , nanotechnology , particle (ecology) , work (physics) , materials science , environmental science , chemistry , mechanical engineering , optics , physics , combustion , engineering , geology , telecommunications , oceanography , scanning transmission electron microscopy , transmission electron microscopy , wireless

Summary The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5–6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our optimal process reduced the time required by over 70% in comparison to a typical procedure, whilst producing models with no appreciable decrease in quality.

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