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Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography
Author(s) -
HAFFNERSTATON E.,
LA ROCCA A.,
FAY M.W.
Publication year - 2018
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12680
Subject(s) - soot , characterization (materials science) , electron tomography , tomography , throughput , process engineering , nanoparticle , computer science , nanotechnology , particle (ecology) , work (physics) , materials science , environmental science , chemistry , mechanical engineering , optics , physics , combustion , engineering , geology , telecommunications , oceanography , scanning transmission electron microscopy , transmission electron microscopy , wireless
Summary The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5–6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our optimal process reduced the time required by over 70% in comparison to a typical procedure, whilst producing models with no appreciable decrease in quality.