Premium
Chemical imaging of a Symbiodinium sp . cell using synchrotron infrared microspectroscopy: a feasibility study
Author(s) -
GORDON B.R.,
MARTIN D.E.,
BAMBERY K.R.,
MOTTI C.A.
Publication year - 2018
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12658
Subject(s) - symbiodinium , zooxanthellae , biology , symbiosis , synchrotron radiation , anthozoa , coral reef , ecology , chemistry , physics , bacteria , genetics , optics
Summary The symbiotic relationship between corals and Symbiodinium spp . is the key to the success and survival of coral reef ecosystems the world over. Nutrient exchange and chemical communication between the two partners provides the foundation of this key relationship, yet we are far from a complete understanding of these processes. This is due, in part, to the difficulties associated with studying an intracellular symbiosis at the small spatial scales required to elucidate metabolic interactions between the two partners. This feasibility study, which accompanied a more extensive investigation of fixed Symbiodinium cells (data unpublished), examines the potential of using synchrotron radiation infrared microspectroscopy (SR‐IRM) for exploring metabolite localisation within a single Symbiodinium cell. In doing so, three chemically distinct subcellular regions of a single Symbiodinium cell were established and correlated to cellular function based on assignment of diagnostic chemical classes.