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A 3D imaging and visualization workflow, using confocal microscopy and advanced image processing for brachyuran crab larvae
Author(s) -
KAMANLI S.A.,
KIHARA T.C.,
BALL A.D.,
MORRITT D.,
CLARK P.F.
Publication year - 2017
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12540
Subject(s) - confocal , visualization , confocal microscopy , preprocessor , microscope , confocal laser scanning microscope , microscopy , workflow , confocal laser scanning microscopy , computer science , rendering (computer graphics) , computer vision , artificial intelligence , biomedical engineering , biology , optics , microbiology and biotechnology , engineering , physics , database
Summary Confocal laser scanning microscopy is an excellent tool for nondestructive imaging of arthropods and can provide detailed information on morphology including fine surface detail. A methodology is presented here for the visualization by confocal microscopy of arthropods, using brachyuran crab zoeal stages as examples and postprocessing techniques derived from micro‐CT protocols to improve the final images. This protocol is divided into description of the preprocessing steps (cleaning, staining, digesting and mounting), confocal laser scanning microscopy and data visualization using open‐source, freeware programs ImageJ and Drishti. The advantages of using ImageJ to standardize stack data and Drishti for surface rendering are discussed. The methodology has been comprehensively tested using data acquired from all four brands of confocal microscope (Leica, Nikon, Olympus and Zeiss).