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Advanced digital image analysis method dedicated to the characterization of the morphology of filamentous fungus
Author(s) -
HARDY N.,
MOREAUD M.,
GUILLAUME D.,
AUGIER F.,
NIENOW A.,
BÉAL C.,
BEN CHAABANE F.
Publication year - 2017
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12523
Subject(s) - filamentous fungus , trichoderma reesei , fungus , digital image analysis , viscosity , biological system , fermentation , segmentation , morphology (biology) , biology , materials science , computer science , microbiology and biotechnology , artificial intelligence , botany , computer vision , food science , composite material , biochemistry , cellulose , genetics , gene , cellulase
Summary Filamentous fungi have a complex morphology that induces fermentation process development issues, as a consequence of viscosity increase and diffusion limitations. In order to better understand the relationship between viscosity changes and fungus morphology during fermentations of Trichoderma reesei , an accurate image analysis method has been developed to provide quantitative and representative data for morphological analysis. This method consisted of a new algorithm called FACE that allowed sharp images to be created at all positions, segmentation of fungus, and morphological analysis using skeleton and topological approaches. It was applied and validated by characterizing samples of an industrial strain of Trichoderma reesei that had or had not been exposed to an extreme shear stress. This method allowed many morphological characteristics to be identified, among which nine relevant criteria were extracted, regarding the impact of shear stress on the fungus and on the viscosity of the fermentation medium.