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Precise observation of C. elegans dynamic behaviours under controlled thermal stimulus using a mobile phone–based microscope
Author(s) -
YOON T.,
SHIN D.M.,
KIM S.,
LEE S.,
LEE T.G.,
KIM K.
Publication year - 2017
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12513
Subject(s) - microscope , mobile phone , phone , caenorhabditis elegans , computer science , optical microscope , stimulus (psychology) , thermal , incubator , computer vision , biology , materials science , telecommunications , optics , physics , scanning electron microscope , psychology , linguistics , philosophy , biochemistry , meteorology , gene , microbiology and biotechnology , composite material , psychotherapist
Summary We investigated the temperature‐dependent locomotion of Caenorhabditis elegans by using the mobile phone–based microscope. We developed the customized imaging system with mini incubator and smartphone to effectively control the thermal stimulation for precisely observing the temperature‐dependent locomotory behaviours of C. elegans . Using the mobile phone–based microscope, we successfully followed the long‐term progress of specimens of C. elegans in real time as they hatched and explored their temperature‐dependent locomotory behaviour. We are convinced that the mobile phone–based microscope is a useful device for real time and long‐term observations of biological samples during incubation, and can make it possible to carry out live observations via wireless communications regardless of location. In addition, this microscope has the potential for widespread use owing to its low cost and compact design.

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