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Bayesian model for detection and classification of meningioma nuclei in microscopic images
Author(s) -
WIRJADI O.,
KIM Y.J.,
STECH F.,
BONFERT L.,
WAGNER M.
Publication year - 2017
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12471
Subject(s) - computer science , segmentation , artificial intelligence , benchmark (surveying) , deconvolution , software , pattern recognition (psychology) , bayesian probability , image segmentation , computer vision , process (computing) , set (abstract data type) , digital pathology , image (mathematics) , algorithm , geodesy , programming language , geography , operating system
Summary Image segmentation aims to determine structures of interest inside a digital picture in biomedical sciences. State‐of‐the art automatic methods however still fail to provide the segmentation quality achievable by humans who employ expert knowledge and use software to mark target structures on an image. Manual segmentation is time‐consuming, tedious and suffers from interoperator variability, thus not serving the requirements of daily use well. Therefore, the approach presented here abandons the goal of full‐fledged segmentation and settles for the localization of circular objects in photographs (10 training images and 20 testing images with several hundreds of nuclei each). A fully trainable softcore interaction point process model was hence fit to the most likely locations of nuclei of meningioma cells. The Broad Bioimage Benchmark Collection/SIMCEP data set of virtual cells served as controls. A ‘colour deconvolution’ algorithm was integrated to determine (based on anti‐Ki67 immunohistochemistry) which real cells might have the potential to proliferate. In addition, a density parameter of the underlying Bayesian model was estimated. Immunohistochemistry results were ‘simulated'for the virtual cells. The system yielded true positive (TP) rates in the detection and classification of real nuclei and their virtual counterparts. These hits outnumbered those obtained from the public domain image processing software ImageJ by 10%. The method introduced here can be trained to function not only in medicine and morphology‐based systems biology but in other application domains as well. The algorithm lends itself to an automated approach that constitutes a valuable tool which is easy to use and generates acceptable results quickly.