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Third‐harmonic generation imaging of breast tissue biopsies
Author(s) -
LEE WOOWON,
KABIR MOHAMMAD M.,
EMMADI RAJYASREE,
TOUSSAINT KIMANI C.
Publication year - 2016
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12427
Subject(s) - microscopy , pathology , h&e stain , biopsy , bright field microscopy , breast tissue , breast biopsy , medicine , staining , mammography , breast cancer , cancer
Summary We demonstrate for the first time the imaging of unstained breast tissue biopsies using third‐harmonic generation (THG) microscopy. As a label‐free imaging technique, THG microscopy is compared to phase contrast and polarized light microscopy which are standard imaging methods for breast tissues. A simple feature detection algorithm is applied to detect tumour‐associated lymphocyte rich regions in unstained breast biopsy tissue and compared with corresponding regions identified by a pathologist from bright‐field images of hematoxylin and eosin stained breast tissue. Our results suggest that THG imaging holds potential as a complementary technique for analysing breast tissue biopsies.