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Serial block face scanning electron microscopy and the reconstruction of plant cell membrane systems
Author(s) -
KITTELMANN M.,
HAWES C.,
HUGHES L.
Publication year - 2016
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12424
Subject(s) - microscopy , scanning electron microscope , electron microscope , microscope , electron tomography , conventional transmission electron microscope , materials science , transmission electron microscopy , scanning transmission electron microscopy , biomedical engineering , optics , nanotechnology , physics , medicine , composite material
Summary Serial block face imaging with the scanning electron microscope has been developed as an alternative to serial sectioning and transmission electron microscopy for the ultrastructural analysis of the three‐dimensional organization of cells and tissues. An ultramicrotome within the microscope specimen chamber permits sectioning and imaging to a depth of many microns within resin‐embedded specimens. The technology has only recently been adopted by plant microscopists and here we describe some specimen preparation procedures suitable for plant tissue, suggested microscope imaging parameters and discuss the software required for image reconstruction and analysis.