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Sample holder for axial rotation of specimens in 3D microscopy
Author(s) -
BRUNS T.,
SCHICKINGER S.,
SCHNECKENBURGER H.
Publication year - 2015
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12263
Subject(s) - light sheet fluorescence microscopy , microscopy , rotation (mathematics) , optics , context (archaeology) , optical microscope , microscope , materials science , perpendicular , sample (material) , physics , scanning confocal electron microscopy , scanning electron microscope , computer science , artificial intelligence , mathematics , geology , geometry , paleontology , thermodynamics
Summary In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three‐dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an axis perpendicular to the light path of the microscope. Thus, images can be put into a defined multidimensional context, enabling reliable three‐dimensional reconstructions. The device can be easily adapted to a great variety of common light microscopes and is suitable for various applications in science, education and industry, where the observation of three‐dimensional specimens is essential. Fluorescence z ‐projection images of copepods and ixodidae ticks at different rotation angles obtained by confocal laser scanning microscopy and light sheet fluorescence microscopy are reported as representative results.