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Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy
Author(s) -
NIE Y.T.,
WU Y.,
FU F.M.,
LIAN Y.E.,
ZHUO S.M.,
WANG C.,
CHEN J.X.
Publication year - 2015
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12219
Subject(s) - fibroadenoma , microscopy , multiphoton fluorescence microscope , pathology , human breast , fluorescence microscope , stroma , materials science , invagination , two photon excitation microscopy , biomedical engineering , fluorescence , medicine , anatomy , optics , immunohistochemistry , cancer cell , physics , cancer , breast cancer
Summary Multiphoton microscopy has become a novel biological imaging technique that allows cellular and subcellular microstructure imaging based on two‐photon excited fluorescence and second harmonic generation. In this work, we used multiphoton microscopy to obtain the high‐contrast images of human normal breast tissue and two main histologic types of fibroadenoma (intracanalicular, pericanalicular). Moreover, quantitative image analysis was performed to characterize the changes of collagen morphology (collagen content, collagen orientation). The results show that multiphoton microscopy combined with quantitative method has the ability to identify the characteristics of fibroadenoma including changes of the duct architecture and collagen morphology in stroma. With the advancement of multiphoton microscopy, we believe that the technique has great potential to be a real‐time histopathological diagnostic tool for intraoperative detection of fibroadenoma in the future.