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Biological applications of cryo‐soft X‐ray tomography
Author(s) -
DUKE E.,
DENT K.,
RAZI M.,
COLLINSON L. M.
Publication year - 2014
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12139
Subject(s) - microscope , biological specimen , optics , resolution (logic) , biological imaging , microscopy , x ray , tomography , electron microscope , materials science , physics , computer science , artificial intelligence , fluorescence
Summary X‐rays are used for imaging many different types of biological specimen, ranging from live organisms to the individual cells and proteins from which they are made. The level of detail achieved as a result of the imaging varies depending on both the sample and the technique used. One of the most recent technical developments in X‐ray imaging is that of the soft X‐ray microscope, designed to allow the internal structure of individual biological cells to be explored. With a field of view of ∼10–20 × ∼10–20 μm, a penetration depth of ∼10 μm and a resolution of ∼40 nm 3 , the soft X‐ray microscope neatly fits between the imaging capabilities of light and electron microscopes.