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A versatile sample holder for single plane illumination microscopy
Author(s) -
DESMAISON ANNAÏCK,
LORENZO CORINNE,
ROUQUETTE JACQUES,
DUCOMMUN BERNARD,
LOBJOIS VALÉRIE
Publication year - 2013
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.12051
Subject(s) - sample (material) , microscopy , computer science , materials science , video microscopy , sample preparation , nanotechnology , optics , chemistry , physics , chromatography , biology , microbiology and biotechnology
Summary Single Plane Illumination Microscopy is an emerging and powerful technology for live imaging of whole living organisms. However, sample handling that relies on specimen embedding in agarose or gel is often a key limitation, especially for time‐lapse monitoring. To address this issue, we developed a new concept for a holder device allowing us to prepare a sample container made of hydrogel. The production process of this holder is based on 3D printing of both a frame and casting devices. The simplicity of production and the advantages of this versatile new sample holder are shown with time‐lapse recording of multicellular tumour spheroid growth. More importantly, we also show that cell division is not impaired in contrast to what is observed with gel embedding. The benefit of this new holder for other sample types, applications and experiments remains to be evaluated, but this innovative concept of fully customizable sample holder preparation potentially represents a major step forward to facilitate the large diffusion of single plane illumination microscopy technology.