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Development of an efficient plant dual cytosine and adenine editor
Author(s) -
Xu Rongfang,
Kong Fanna,
Qin Ruiying,
Li Juan,
Liu Xiaoshuang,
Wei Pengcheng
Publication year - 2021
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.13146
Subject(s) - dual (grammatical number) , cytosine , base (topology) , computer science , computational biology , dual purpose , biology , genetics , engineering , dna , mathematics , linguistics , philosophy , mechanical engineering , mathematical analysis
Summary An enhanced CDA‐like (eCDAL) was established from Japanese lamprey CDA1‐like 4 to achieve a high editing frequency in a broad region as a C‐terminal cytosine base editors (CT‐CBE). Then, a novel plant dual‐base editor version 1(pDuBE1) was developed by integrating TadA‐8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A‐to‐G and C‐to‐T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.