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Genome editing for plant research and crop improvement
Author(s) -
Zhan Xiangqiang,
Lu Yuming,
Zhu JianKang,
Botella Jose Ramon
Publication year - 2021
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.13063
Subject(s) - crispr , genome editing , indel , biology , palindrome , epigenome , computational biology , genetics , gene , gene expression , dna methylation , genotype , single nucleotide polymorphism
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state‐of‐the‐art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control.