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The Class III peroxidase gene OsPrx30 , transcriptionally modulated by the AT‐hook protein OsATH1, mediates rice bacterial blight‐induced ROS accumulation
Author(s) -
Liu Hao,
Dong Shuangyu,
Li Ming,
Gu Fengwei,
Yang Guili,
Guo Tao,
Chen Zhiqiang,
Wang Jiafeng
Publication year - 2021
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.13040
Subject(s) - oryza sativa , xanthomonas oryzae pv. oryzae , peroxidase , gene , biology , reactive oxygen species , xanthomonas oryzae , transcription factor , promoter , microbiology and biotechnology , bacterial blight , transcription (linguistics) , botany , biochemistry , enzyme , gene expression , linguistics , philosophy
Class III peroxidases (CIII Prxs) play critical roles in plant immunity by scavenging reactive oxygen species (ROS). However, the functions of CIII Prxs in rice ( Oryza sativa L.) immunity are largely unexplored. Here, we report a Prx precursor, OsPrx30, that is responsive to the bacterial blight Xanthomonas oryzae pv. oryzae ( Xoo ). OsPrx30 was primarily expressed in rice roots, leaves, and stems, and its protein product was mainly localized at the endoplasmic reticulum. Overexpression of OsPrx30 enhanced the plant's susceptibility to Xoo by maintaining a high level of peroxidase (POD) activity and reducing the content of H 2 O 2 , whereas depletion of OsPrx30 had the opposite effects. Furthermore, we identified an AT‐hook transcription factor, OsATH1, that is specifically bound to the OsPrx30 promoter. As observed in plants overexpressing OsPrx30 , depletion of OsATH1 enhanced susceptibility to Xoo . Finally, we demonstrated that depletion of OsATH1 increased histone H3 acetylation at the AT‐rich region of the OsPrx30 promoter. Taken together, these results reveal a mechanism underlying the POD‐induced natural resistance to bacterial diseases and suggest a model for transcription regulation of Prx genes in rice.