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Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae
Author(s) -
Yin Zhiyuan,
Wang Nan,
Pi Lei,
Li Lei,
Duan Weiwei,
Wang Xiaodan,
Dou Daolong
Publication year - 2021
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.13031
Subject(s) - nicotiana benthamiana , verticillium dahliae , verticillium wilt , biology , oomycete , plant disease resistance , fungal protein , microbiology and biotechnology , botany , gene , pathogen , genetics , saccharomyces cerevisiae
Verticillium wilt diseases caused by the soil‐borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethylene‐inducing xylanase (EIX)‐like protein, VdEIX3, from V. dahliae , which exhibits immunity‐inducing activity in Nicotiana benthamiana . In vitro ‐purified VdEIX3 can induce strong oxidative burst, activate the expression of defense‐related genes, and increase resistance against oomycete and fungal pathogens in N. benthamiana . VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N. benthamiana , which form a new type of EIX protein family that is distinct from the known EIX proteins. A leucine‐rich repeat receptor‐like protein, NbEIX2, regulates the perception of VdEIX3 in N. benthamiana . Our results demonstrate that VdEIX3 is a novel EIX‐like protein that can be recognized by N. benthamiana NbEIX2, and also suggest that NbEIX2 is a promising receptor‐like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.