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FKF1 F‐box protein promotes flowering in part by negatively regulating DELLA protein stability under long‐day photoperiod in Arabidopsis
Author(s) -
Yan Jindong,
Li Xinmei,
Zeng Bingjie,
Zhong Ming,
Yang Jiaxin,
Yang Piao,
Li Xin,
He Chongsheng,
Lin Jianzhong,
Liu Xuanming,
Zhao Xiaoying
Publication year - 2020
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12971
Subject(s) - arabidopsis , photoperiodism , gibberellin , biology , f box protein , microbiology and biotechnology , repressor , signal transduction , genetics , ubiquitin ligase , ubiquitin , botany , mutant , gene , transcription factor
FLAVIN‐BINDING KELCH REPEAT F‐BOX 1 ( FKF1 ) encodes an F‐box protein that regulates photoperiod flowering in Arabidopsis under long‐day conditions (LDs). Gibberellin (GA) is also important for regulating flowering under LDs. However, how FKF1 and the GA pathway work in concert in regulating flowering is not fully understood. Here, we showed that the mutation of FKF1 could cause accumulation of DELLA proteins, which are crucial repressors in GA signaling pathway, thereby reducing plant sensitivity to GA in flowering. Both in vitro and in vivo biochemical analyses demonstrated that FKF1 directly interacted with DELLA proteins. Furthermore, we showed that FKF1 promoted ubiquitination and degradation of DELLA proteins. Analysis of genetic data revealed that FKF1 acted partially through DELLAs to regulate flowering under LDs. In addition, DELLAs exerted a negative feedback on FKF1 expression. Collectively, these findings demonstrate that FKF1 promotes flowering partially by negatively regulating DELLA protein stability under LDs, and suggesting a potential mechanism linking the FKF1 to the GA signaling DELLA proteins.