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The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis
Author(s) -
Zeb Qudsia,
Wang Xiaohan,
Hou Congcong,
Zhang Xiwen,
Dong Mengqi,
Zhang Sisi,
Zhang Qian,
Ren Zhijie,
Tian Wang,
Zhu Huifen,
Li Legong,
Liu Liangyu
Publication year - 2020
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12890
Subject(s) - root hair , phenocopy , bimolecular fluorescence complementation , mutant , calmodulin , polarity (international relations) , complementation , microbiology and biotechnology , biophysics , cytosol , arabidopsis , chemistry , calcium , patch clamp , arabidopsis thaliana , biology , biochemistry , yeast , receptor , gene , enzyme , organic chemistry , cell
Oscillations in cytosolic free calcium determine the polarity of tip‐growing root hairs. The Ca 2+ channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca 2+ concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two‐hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF‐hand domain of CaM7 specifically interacts with the cytosolic C‐terminal domain of CNGC14. A two‐electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14‐mediated Ca 2+ influx, suggesting that CaM7 negatively regulates CNGC14‐mediated calcium signaling. Furthermore, CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca 2+ concentrations. We, thus, identified CaM7‐CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip‐focused Ca 2+ signal.