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Genetic mapping of folate QTLs using a segregated population in maize
Author(s) -
Guo Wenzhu,
Lian Tong,
Wang Baobao,
Guan Jiantao,
Yuan Dong,
Wang Huan,
Safiul Azam Fardous Mohammad,
Wan Xing,
Wang Weixuan,
Liang Qiuju,
Wang Haiyang,
Tu Jinxing,
Zhang Chunyi,
Jiang Ling
Publication year - 2019
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12811
Subject(s) - biology , population , quantitative trait locus , genetics , agronomy , evolutionary biology , gene , demography , sociology
As essential B vitamin for humans, folates accumulation in edible parts of crops, such as maize kernels, is of great importance for human health. But its breeding is always limited by the prohibitive cost of folate profiling. The molecular breeding is a more executable and efficient way for folate fortification, but is limited by the molecular knowledge of folate regulation. Here we report the genetic mapping of folate quantitative trait loci (QTLs) using a segregated population crossed by two maize lines, one high in folate (GEMS31) and the other low in folate (DAN3130). Two folate QTLs on chromosome 5 were obtained by the combination of F 2 whole‐exome sequencing and F 3 kernel‐folate profiling. These two QTLs had been confirmed by bulk segregant analysis using F 6 pooled DNA and F 7 kernel‐folate profiling, and were overlapped with QTLs identified by another segregated population. These two QTLs contributed 41.6% of phenotypic variation of 5‐formyltetrahydrofolate, the most abundant storage form among folate derivatives in dry maize grains, in the GEMS31×DAN3130 population. Their fine mapping and functional analysis will reveal details of folate metabolism, and provide a basis for marker‐assisted breeding aimed at the enrichment of folates in maize kernels.