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A common metabolomic signature is observed upon inoculation of rice roots with various rhizobacteria
Author(s) -
Valette Marine,
Rey Marjolaine,
Gerin Florence,
Comte Gilles,
WisniewskiDyé Florence
Publication year - 2020
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12810
Subject(s) - rhizobacteria , biology , secondary metabolite , metabolomics , bacteria , inoculation , microbial inoculant , proteobacteria , transcriptome , pathogen , cultivar , gene , metabolite , hydroxycinnamic acid , seedling , metabolome , botany , microbiology and biotechnology , horticulture , rhizosphere , gene expression , biochemistry , genetics , 16s ribosomal rna , bioinformatics , antioxidant
Plant growth‐promoting rhizobacteria (PGPR), whose growth is stimulated by root exudates, are able to improve plant growth and health. Among those, bacteria of the genus Azospirillum were shown to affect root secondary metabolite content in rice and maize, sometimes without visible effects on root architecture. Transcriptomic studies also revealed that expression of several genes involved in stress and plant defense was affected, albeit with fewer genes when a strain was inoculated onto its original host cultivar. Here, we investigated, via a metabolic profiling approach, whether rice roots responded differently and with gradual intensity to various PGPR, isolated from rice or not. A common metabolomic signature of nine compounds was highlighted, with the reduced accumulation of three alkylresorcinols and increased accumulation of two hydroxycinnamic acid amides (HCAA), identified as N ‐ p ‐coumaroylputrescine and N ‐feruloylputrescine. This was accompanied by the increased transcription of two genes involved in the N ‐feruloylputrescine biosynthetic pathway. Interestingly, exposure to a rice bacterial pathogen triggered a reduced accumulation of these HCAA in roots, a result contrasting with previous reports of increased HCAA content in leaves upon pathogen infection. Accumulation of HCAA, that are potential antimicrobial compounds, might be considered as a primary reaction of plant to bacterial perception.