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The F‐box protein HAWAIIAN SKIRT is required for mimicry target‐induced microRNA degradation in Arabidopsis
Author(s) -
Mei Jun,
Jiang Ning,
Ren Guodong
Publication year - 2019
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12761
Subject(s) - mimicry , microrna , biology , gene silencing , molecular mimicry , rna , phenotype , genetics , small rna , arabidopsis , microbiology and biotechnology , gene , ecology , antibody , mutant
Summary Mimicry target‐directed microRNA degradation is widespread and highly conserved among eukaryotes. However, little is known about its mechanism of action. In this letter, by using STTM160 (target mimic of miR160) as a reporter, we show that dysfunction of HAWAIIAN SKIRT (HWS) suppresses the pleiotropic phenotype of STTM160. Small RNA sequencing and Northern blot analyses suggested that HWS only affects a subset of microRNAs. Intriguingly, we identified a stable coexistence of miR160/miR399 and their mimicry targets within the AGO1 complex when HWS is compromised, pointing to a possible role of HWS in the clearance of RNA‐induced silencing complexes associated with mimicry target.