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Arabidopsis ANAC092 regulates auxin‐mediated root development by binding to the ARF8 and PIN4 promoters
Author(s) -
Xi Dandan,
Chen Xu,
Wang Yuxia,
Zhong Ruiling,
He Jianmei,
Shen Jiabin,
Ming Feng
Publication year - 2019
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12735
Subject(s) - auxin , arabidopsis , biology , apical dominance , meristem , transcription factor , chromatin immunoprecipitation , organogenesis , microbiology and biotechnology , lateral root , promoter , plant hormone , mutant , morphogenesis , botany , genetics , gene expression , gene , shoot
Auxin is an important plant hormone that is essential for growth and development due to its effects on organogenesis, morphogenesis, tropisms, and apical dominance. The functional diversity of auxin highlights the importance of its biosynthesis, transport, and associated responses. In this study, we show that a NAC transcription factor, ANAC092 (also named AtNAC2 and ORESARA1), known to positively regulate leaf senescence and contribute to abiotic stress responses, also affects primary root development. Plants overexpressing ANAC092 had altered root meristem lengths and shorter primary roots compared with the wild‐type control. Additionally, expression of the proANAC092::GUS was strongly induced by indole‐3‐acetic acid. Quantitative real‐time RT‐PCR (qRT‐PCR) analysis revealed that the YUCCA2, PIN , and ARF expression levels were downregulated in ANAC092 ‐overexpressing plants. Moreover, yeast one‐hybrid and chromatin immunoprecipitation assays confirmed that ANAC092 binds to the promoters of AUXIN RESPONSE FACTOR 8 ( ARF8 ) and PIN‐FORMED 4 ( PIN4 ). Furthermore, a dual‐luciferase assay indicated that ANAC092 decreases ARF8 and PIN4 promoter activities. We also applied a CRISPR/Cas9 system to mutate ANAC092 . The roots of three of the analyzed mutants were longer than normal. Collectively, our findings indicate that ANAC092 negatively affects root development by controlling the auxin pathway.