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Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis
Author(s) -
Kim Soohong,
Choi Yunjin,
Kwon Chian,
Yun Hye Sup
Publication year - 2019
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12728
Subject(s) - calreticulin , endoplasmic reticulum , tunicamycin , unfolded protein response , arabidopsis , microbiology and biotechnology , translation (biology) , biology , biochemistry , gene , messenger rna , mutant
Summary Excessive demand for translation and protein folding in the endoplasmic reticulum (ER) can cause ER stress in plants. Here, we show that CALRETICULIN 1 (CRT1) and CRT2 are critical components in the accumulation of VESICLE‐ASSOCIATED MEMBRANE PROTEIN 721 (VAMP721) and VAMP722 during ER stress responses. We show that CRT2 interacts with VAMP722 and that CRT1/2 post‐translationally maintain elevated VAMP721/722 levels under ER stress. The greater growth inhibition in VAMP721/722‐deficient plants, induced by tunicamycin, suggests that plants under ER stress maintain physiological homeostasis, at least in part, by regulating VAMP721/722 levels, as VAMP721/722 are known to participate in various biological processes.