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Essential role of NbNOG1 in ribosomal RNA processing
Author(s) -
Guo Jiangbo,
Han Shaojie,
Zhao Jinping,
Xin Cuihua,
Zheng Xiyin,
Liu Yule,
Wang Yan,
Qu Feng
Publication year - 2018
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12691
Subject(s) - ribosome biogenesis , biology , nicotiana benthamiana , ribosome , ribosomal rna , microbiology and biotechnology , biogenesis , yeast , ribosomal protein , rna , saccharomyces cerevisiae , genetics , gene
Summary Nucleolar GTP‐binding protein 1 (NOG1) is a highly conserved GTPase first reported in Trypanosoma as required for ribosome biogenesis. We characterized NbNOG1, a Nicotiana benthamiana NOG1 ortholog sharing more than 45% amino acid identity with Trypanosoma , yeast, and human NOG1. N. benthamiana plants silenced for NbNOG1 were stunted and produced sterile flowers. NbNOG1 is functionally interchangeable with yeast NOG1 (ScNOG1) , rescuing yeast lethality caused by loss of ScNOG1 . Finally, NbNOG1 silencing caused over‐accumulation of pre‐rRNA processing intermediates, and concomitant loss of mature rRNAs. Collectively, these data support a role for NbNOG1 in ribosomal RNA processing.