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Multiplex gene editing in rice with simplified CRISPR‐Cpf1 and CRISPR‐Cas9 systems
Author(s) -
Wang Mugui,
Mao Yanfei,
Lu Yuming,
Wang Zhidan,
Tao Xiaoping,
Zhu JianKang
Publication year - 2018
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12667
Subject(s) - trans activating crrna , crispr , multiplex , genome editing , cas9 , computational biology , biology , gene , nuclease , crispr interference , genetics
Summary We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co‐expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.