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A novel wheat NAC transcription factor, Ta NAC30, negatively regulates resistance of wheat to stripe rust
Author(s) -
Wang Bing,
Wei Jinping,
Song Na,
Wang Ning,
Zhao Jing,
Kang Zhensheng
Publication year - 2018
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12627
Subject(s) - gene silencing , biology , transcription factor , gene , transcription (linguistics) , microbiology and biotechnology , virulence , yeast , genetics , philosophy , linguistics
NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30 , from a cDNA library constructed from wheat ( Triticum aestivum ) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici ( Pst ). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one‐hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C‐terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst . Silencing of TaNAC30 by virus‐induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 . Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30 ‐transformed cells grown on medium containing H 2 O 2 . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat‐ Pst interaction.