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Multigene editing via CRISPR/Cas9 guided by a single‐sgRNA seed in Arabidopsis
Author(s) -
Yu Zhiming,
Chen Qiyuan,
Chen Weiwei,
Zhang Xian,
Mei Fengling,
Zhang Pengcheng,
Zhao Mei,
Wang Xiaohong,
Shi gg,
Jackson Stephen,
Hong Yiguo
Publication year - 2018
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12622
Subject(s) - crispr , genome editing , cas9 , arabidopsis , subgenomic mrna , biology , gene , guide rna , genetics , computational biology , palindrome , mutant
Summary We report that a solo single‐guide RNA (sgRNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR −associated 9 (CRISRP/Cas9) to simultaneously edit multiple genes AtRPL10A , AtRPL10B and AtRPL10C in Arabidopsis . Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off‐target gene‐editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less‐homologous sequences for multiple gene‐editing in plants and other organisms.