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A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway
Author(s) -
Zhang XiaoBo,
Feng BaoHua,
Wang HuiMei,
Xu Xia,
Shi YongFeng,
He Yan,
Chen Zheng,
Sathe Atul Prakash,
Shi Lei,
Wu JianLi
Publication year - 2018
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12613
Subject(s) - biology , mutant , salicylic acid , complementation , genetics , gene , positional cloning , biochemistry
We previously reported a spotted‐leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae . Here, we report the map‐based cloning of the causal gene OsPELOTA (originally termed spl HM47 ). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA , effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over‐expression could rescue the spotted‐leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA‐seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.