Tonoplast targeting of VHA‐a3 relies on a Rab5‐mediated but Rab7‐independent vacuolar trafficking route

Summary Vacuolar trafficking routes and their regulators have recently drawn lots of attention in plant cell biology. A recent study reported the discovery of a plant‐specific vacuolar trafficking route, i.e., a direct ER‐to‐vacuole route, through analysis of VHA‐a3 subcellular targeting, a key component for the tonoplast V‐ATPases. Our recent findings showed that VHA‐a3 targets to the tonoplast through a Rab5‐mediated but Rab7‐independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells.