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Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H 2 evolution
Author(s) -
Chen Mei,
Zhang Jin,
Zhao Lei,
Xing Jiale,
Peng Lianwei,
Kuang Tingyun,
Rochaix JeanDavid,
Huang Fang
Publication year - 2016
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12502
Subject(s) - reactive oxygen species , mixotroph , electron transport chain , photosynthesis , electrochemical gradient , oxygen , biophysics , mutant , oxygen evolution , chemistry , biochemistry , sulfur , proton transport , dcmu , biology , photosystem ii , membrane , heterotroph , electrochemistry , organic chemistry , electrode , bacteria , gene , genetics
Summary We have identified hpm91 , a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H 2 ) for 25 days with more than 30‐fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H 2 production than a previously characterized pgr5 mutant. Physiological and biochemical characterization of hpm91 reveal that the prolonged H 2 production is due to enhanced stability of PSII, which correlates with increased reactive oxygen species (ROS) scavenging capacity during sulfur deprivation. This anti‐ROS response appears to protect the photosynthetic electron transport chain from photo‐oxidative damage and thereby ensures electron supply to the hydrogenase.