Premium
Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination
Author(s) -
Chen Bingxian,
Ma Jun,
Xu Zhenjiang,
Wang Xiaofeng
Publication year - 2016
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12479
Subject(s) - radicle , endosperm , ethephon , germination , cellulase , abscisic acid , coleoptile , lactuca , botany , biology , imbibition , elongation , cellulose , biochemistry , gene , materials science , ultimate tensile strength , metallurgy , ethylene , catalysis
The purpose of this study was to investigate the role of cellulase in endosperm cap weakening and radicle elongation during lettuce ( Lactuca sativa L.) seed germination. The application of abscisic acid (ABA) or ethephon inhibits or promotes germination, respectively, by affecting endosperm cap weakening and radicle elongation. Cellulase activities, and related protein and transcript abundances of two lettuce cellulase genes, LsCEL1 and LsCEL2 , increase in the endosperm cap and radicle prior to radicle protrusion following imbibition in water. ABA or ethephon reduce or elevate, respectively, cellulase activity, and related protein and transcript abundances in the endosperm cap. Taken together, these observations suggest that cellulase plays a role in endosperm cap weakening and radicle elongation during lettuce seed germination, and that the regulation of cellulase in the endosperm cap by ABA and ethephon play a role in endosperm cap weakening. However, the influence of ABA and ethephon on radicle elongation may not be through their effects on cellulase.