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A gain‐of‐function mutation in Msl10 triggers cell death and wound‐induced hyperaccumulation of jasmonic acid in Arabidopsis
Author(s) -
Zou Yan,
Chintamanani Satya,
He Ping,
Fukushige Hirotada,
Yu Liping,
Shao Meiyu,
Zhu Lihuang,
Hildebrand David F.,
Tang Xiaoyan,
Zhou JianMin
Publication year - 2016
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12427
Subject(s) - mutant , microbiology and biotechnology , biology , arabidopsis , jasmonic acid , signal transduction , transcription factor , phenotype , genetic screen , gene , genetics
Jasmonates (JAs) are rapidly induced after wounding and act as key regulators for wound induced signaling pathway. However, what perceives the wound signal and how that triggers JA biosynthesis remains poorly understood. To identify components involved in Arabidopsis wound and JA signaling pathway, we screened for mutants with abnormal expression of a luciferase reporter, which is under the control of a wound‐responsive promoter of an ethylene response factor ( ERF ) transcription factor gene, RAP2.6 (Related to APetala 2.6). The rea1 ( R AP2.6 e xpresser in shoot a pex) mutant constitutively expressed the RAP2.6‐LUC reporter gene in young leaves. Along with the typical JA phenotypes including shorter petioles, loss of apical dominance, accumulation of anthocyanin pigments and constitutive expression of JA response gene, rea1 plants also displayed cell death and accumulated high levels of JA in response to wounding. The phenotype of rea1 mutant is caused by a gain‐of‐function mutation in the C‐terminus of a mechanosensitive ion channel MscS‐like 10 ( MSL10 ). MSL10 is localized in the plasma membrane and is expressed predominantly in root tip, shoot apex and vascular tissues. These results suggest that MSL10 is involved in the wound‐triggered early signal transduction pathway and possibly in regulating the positive feedback synthesis of JA.