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The alteration in the architecture of a T‐DNA insertion rice mutant osmtd1 is caused by up‐regulation of MicroRNA156f
Author(s) -
Liu Qing,
Shen Gezhi,
Peng Keqin,
Huang Zhigang,
Tong Jianhua,
Kabir Mohammed Humayun,
Wang Jianhui,
Zhang Jingzhe,
Qin Genji,
Xiao Langtao
Publication year - 2015
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12340
Subject(s) - mutant , biology , gene , tiller (botany) , oryza sativa , wild type , phenotype , genetics , dna , mutation , microbiology and biotechnology , botany
Plant architecture is an important factor for crop production. Some members of microRNA156 ( miR156 ) and their target genes SQUAMOSA Promoter‐Binding Protein‐Like ( SPL ) were identified to play essential roles in the establishment of plant architecture. However, the roles and regulation of miR156 is not well understood yet. Here, we identified a T‐DNA insertion mutant Osmtd1 ( Oryza sativa multi‐tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T‐DNA insertion in Osmtd1 . Further analysis revealed that the T‐DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and OsmiR156f was significantly upregulated in Osmtd1 . Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of OsmiR156f in wild‐type rice phenocopied Osmtd1 . We showed that the expression of OsSPL3 , OsSPL12 , and OsSPL14 were significantly downregulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3 , OsSPL12 , and OsSPL14 were possibly direct target genes of OsmiR156f . Our results suggested that OsmiR156f controlled plant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein.