Premium
Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora
Author(s) -
Hirakawa Yumi,
Nomura Toshihisa,
Hasezawa Seiichiro,
Higaki Takumi
Publication year - 2015
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12304
Subject(s) - vacuole , programmed cell death , microbiology and biotechnology , biology , plant cell , lysis , cell , nicotiana tabacum , biochemistry , apoptosis , cytoplasm , gene
Vacuoles are suggested to play crucial roles in plant defense‐related cell death. During programmed cell death, previous live cell imaging studies have observed vacuoles to become simpler in structure and have implicated this simplification as a prelude to the vacuole's rupture and consequent lysis of the plasma membrane. Here, we examined dynamics of the vacuole in cell cycle‐synchronized tobacco BY‐2 ( Nicotiana tabacum L. cv. Bright Yellow 2) cells during cell death induced by application of culture filtrates of Erwinia carotovora . The filtrate induced death in about 90% of the cells by 24 h. Prior to cell death, vacuole shape simplified and endoplasmic actin filaments disassembled; however, the vacuoles did not rupture until after plasma membrane integrity was lost. Instead of facilitating rupture, the simplification of vacuole structure might play a role in the retrieval of membrane components needed for defense‐related cell death.