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A haplotype block associated with thousand‐kernel weight on chromosome 5DS in common wheat ( Triticum aestivum L.)
Author(s) -
Wang Yuquan,
Hao Chenyang,
Zheng Jun,
Ge Hongmei,
Zhou Yang,
Ma Zhengqiang,
Zhang Xueyong
Publication year - 2015
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12294
Subject(s) - germplasm , biology , linkage disequilibrium , haplotype , heritability , locus (genetics) , genetics , population , common wheat , allele , chromosome , gene , botany , demography , sociology
Abstract Spike number per unit area, number of grains per spike, and thousand‐kernel weight (TKW) are important yield components for wheat ( Triticum aestivum L.). TKW has the highest heritability among the three components. We validated 27 simple sequence repeat (SSR) loci associated with TKW in an F 2:5 breeding population grown in four environments. A cfd78 265bp marker on chromosome 5DS showed the strongest association with TKW and had a significantly positive effect on TKW compared to allele cfd78 259bp , with mean increases of 5.17, 3.63, 4.11, and 5.16 g in the four environments. Markers cfd67 and cfd40 flanking cfd78 also showed significantly positive associations with TKW with increases of 5.11, 3.29, 4.31, and 4.50 g for cfd67 205 , and 4.98, 3.49, 4.06, and 4.84 g for cfd40 187 compared with cfd67 203 and cfd40 190 in the four environments, respectively. A major quantitative trait locus for TKW spanning 2.94 cM on chromosome 5DS was detected by association mapping. Strong linkage disequilibrium (LD) ( r 2 > 0.2) was detected among the three linked markers, which formed three haplotype blocks in the F 2:5 breeding population. Mean TKW differences between HapB ‐I and HapB ‐II were 5.80, 4.41, 4.02, and 5.06 g in the four environments, respectively. Moreover, significant LD was detected only between cfd78 and cfd67 and between cfd67 and cfd40 in a germplasm collection. This study provides a base for cloning genes related to TKW on chromosome 5DS.