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ZmGns, a maize class I β‐1,3‐glucanase, is induced by biotic stresses and possesses strong antimicrobial activity
Author(s) -
Xie YuRong,
Raruang Yenjit,
Chen ZhiYuan,
Brown Robert L.,
Cleveland Thomas E.
Publication year - 2015
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12286
Subject(s) - pseudomonas syringae , botrytis cinerea , biology , abiotic component , microbiology and biotechnology , salicylic acid , biotic stress , aspergillus flavus , chitinase , abiotic stress , bacteria , pathogenesis related protein , pathogen , gene , gene expression , botany , genetics , paleontology
Plant β‐1,3‐glucanases are members of the pathogenesis‐related protein 2 (PR‐2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differentially expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize ( Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns . ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in all tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus . ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo , ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis . Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as well as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense.