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An auxin‐responsive endogenous peptide regulates root development in Arabidopsis
Author(s) -
Yang Fengxi,
Song Yu,
Yang Hao,
Liu Zhibin,
Zhu Genfa,
Yang Yi
Publication year - 2014
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/jipb.12178
Subject(s) - auxin , meristem , arabidopsis , biology , lateral root , endogeny , microbiology and biotechnology , rna interference , mutant , transgene , cotyledon , wild type , root hair , botany , shoot , gene , biochemistry , rna
Auxin plays critical roles in root formation and development. The components involved in this process, however, are not well understood. Here, we newly identified a peptide encoding gene, auxin‐responsive endogenous polypeptide 1 ( AREP1 ), which is induced by auxin, and mediates root development in Arabidopsis . Expression of AREP1 was specific to the cotyledon and to root and shoot meristem tissues. Amounts of AREP1 transcripts and AREP1‐green fluorescent protein fusion proteins were elevated in response to indoleacetic acid treatment. Suppression of AREP1 through RNAi silencing resulted in reduction of primary root length, increase of lateral root number, and expansion of adventitious roots, compared to the observations in wild‐type plants in the presence of auxin. By contrast, transgenic plants overexpressing AREP1 showed enhanced growth of the primary root under auxin treatment. Additionally, root morphology, including lateral root number and adventitious roots, differed greatly between transgenic and wild‐type plants. Further analysis indicated that the expression of auxin‐responsive genes, such as IAA3 , IAA7 , IAA17 , GH3.2 , GH3.3 , and SAUR‐AC1 , was significantly higher in AREP1 RNAi plants, and was slightly lower in AREP1 overexpressing plants than in wild‐type plants. These results suggest that the novel endogenous peptide AREP1 plays an important role in the process of auxin‐mediated root development.